Determination of Arsenic in Minor Cereals (Barley, Foxtail Millet, Proso-Millet, Finger- Millet, Pearl -Millet, Buckwheat. Oat, Quinoa And Sorghum) in Gazipur, Bangladesh

Authors

• Most. Shanaj Parvin, Fahmida Akhter, Mubarak Ali Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh
• MdEhsanul Haque University of North Dakota, Grand Forks, USA
• Most. Shanaj Parvin Faculty of Natural Science, Leibniz University Hannover, Germany

Abstract

Cereal grains are the most important source of the world’s total food and also the major accumulator of toxic arsenic. Arsenic distributes in the environment via contaminated ground water which is used as drinking water and irrigated in the crop field. Chronic arsenic toxicity can be lethal for liver, kidney, bladder, lung, skin. Skin hyperpigmentation and hyperkeratosis are common exposure related phenotypes. Therefore, it's high time to produce ideal cereals that must contain a lower amount of arsenic and ensure safety for all kinds of lives. Ensuring safe food is one of the objectives of sustainable development goal-2 (SDG-2). With this view in mind, the Plant Breeding Division of BARI currently working with nine minor kinds of cereal included Barley, Foxtail millet, Proso-millet, Finger-millet, Pearl-millet, Buckwheat, Oat, Quinoa, and BARI-Sorghum 1 were undertaken for biochemical analysis of arsenic on their leaves. The biochemical analysis of these nine kinds of minor cerealsshowed negligible amount of arsenic on leaves. Further analysis in other location in Bangladesh might bring the overall picture of arsenic uptake in minor cereals.

Introduction

Cereals are a staple food for many people around the world including Bangladesh; however, they are also a major dietary source of toxic arsenic (As). Most agricultural lands of Bangladesh are contaminated with arsenic (59 out of 64 districts are arsenic contaminated according to IAEA, 2002), which can be accumulated to high levels in the grains of cereals cultivated in these regions, posing serious health risks to consumers.  Arsenic has two forms such as trivalent (ASIII) and pentavalent (AsV), thus it has larger atomic radius, more electron clouds, relative higher reaction affinity (ASIII) to thiol group/sulfhydryl group (-SH) groups than other divalent cations (Most and Papenbrock 2015). As such this notorious heavy metal being efficiently absorbed in skin, lung, kidney, liver, and bladder than any other heavy metals. Though, the level of arsenic contamination and its consequence has been well studied in rice, however, a similar study has not been performed in other cereals (like wheat, maize, barley, and foxtail millet, etc) despite their increasing trend of production and end-use. Hence, to save the nation, it is imperative to develop cereals that will contain reduced levels of arsenic. Genetic engineering strategies could be employed to develop a variety that will retain a lower amount of toxic arsenic. Therefore, the level of arsenic in the existing cereals has to be determined as a starting point towards lower arsenic variety development. Thus, the arsenic content in the minor cereals available in PBD and grown in Joydebpur soil (Barley, Foxtail millet, Proso-millet, Finger-millet, Pearl-millet, Buckwheat, Oat, Quinoa, and BARI–Sorghum 1) were determined in this experiment. The primary arsenic status of these cereals may have given clues for designing the appropriate breeding program in the future.

Materials and Methods

The leaves of available nine kinds minor of cereal (Barley, Foxtail millet, Proso-millet, Finger- millet, Pearl-millet, Buckwheat. Oat, Quinoa, and BARI–Sorghum 1) were collected from Joydebpur experimental field to their total arsenic accumulation. Before elemental analysis, the leaves samples were oven-dried at a constant temperature of 65° C for 10 days. Subsequently, dried leaves were grounded into fine powder for arsenic level determination. Simultaneously, a soil sample from the same location was collected and subjected to arsenic level determination. The elemental analysis was performed by the soil science division of BARI.

Results and Discussion

The chemical analysis of nine kinds of cereal has shown arsenic accumulation in their leaves in table 1. The unit value of arsenic was expressed in ppm (mg l^-1 or mg kg^-1). The table depicted that total arsenic accumulation of all the minor cereals was remaining an acceptable limit (1-920 µg kg^-1). The acceptable limit of arsenic in the terrestrial plant was determined less than 10 mg kg-1 under normal conditions (Matschullat, 2000). Several plants contain arsenic in the following order: cabbage

(0.020 – 0.050 mg kg^-1) < carrots (0.040 – 0.080) < grass (0.020 – 0.160) < potatoes (0.020- 0.200) < lettuce (0.020 – 0.250) < mosses and lichens (0.26) < ferns (1.3) (Matschullat, 2000). Comparing the arsenic content in the above-mentioned crops with the nine minor kinds of cereal of PBD, all the cereals have shown a negligible amount of arsenic. Although it has been shown that BARI Kaon-1 contains an increasing amount of arsenic comparing with others, it holds arsenic an acceptable limit; because, an average toxicity threshold of 40 mg kg^-1 was established for crop plants (Sheppard et al., 1992). The soil-arsenic analysis was shown 3 mg kg-1 which is also negligible that indicates corresponding sites are not contaminated by arsenic. According to Adriano (1986), soil-arsenic concentration typically varies from below 10 mg kg^-1 in non-contaminated soils to as high as 30,000 mg kg^-1 in contaminated soils (Vaughan, 1993). So, it is pragmatic when the soil has less amount of arsenic and crop grown in that particular soil must have less amount of arsenic. In the current experimental condition, it is not possible to say that the crops have less capacity for arsenic accumulation. It could possible they can extract higher arsenic in contaminated soil.

Table 1. Total arsenic analysis of nine kinds of minor cereal and one soil sample

Conclusion

The cereals grown in Joydeppur's experimental field have shown non-contamination by arsenic. Therefore, it can be said that seeds produced in Joydebpur experimental field and distributed to farmers and other organizations are free of arsenic. Safe food consumption is not only significant for human but also require for other animals, therefore, ensuring safe food for all kind of lives is one of the objectives of sustainable development goal-2 (SDG-2). The biochemical analysis needs to be extended for crops that are growing in arsenic-contaminated sites, typically Chandpur, Munshiganj, Gopalganj, Madaripur, Noakhali, Satkhira, Comilla, Meherpur, Bagerhat and Khulna region which are the high arsenic-contaminated zone. In addition, similar analysis can be carried out in control conditions where crops will be subjected to varying concentrations of arsenic. The expected output could help to developing an arsenic resistance crop.

References

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